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anti par3  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti par3
    Anti Par3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+par3/PAR-3+Antibody/pm41910362-285-10-34
    Average 93 stars, based on 98 article reviews
    anti par3 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein
    Article Snippet: .. The cell lysates were centrifugation at 12,000×g for 15 min at 4°C and then incubated with anti-Par3 or anti-14-3-3ζ antibody covalently coupled to protein A/G-agarose beads (Santa Cruz Biotechnology, USA) at 4°C overnight. ..

    Incubation:

    Article Title: Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein
    Article Snippet: .. The cell lysates were centrifugation at 12,000×g for 15 min at 4°C and then incubated with anti-Par3 or anti-14-3-3ζ antibody covalently coupled to protein A/G-agarose beads (Santa Cruz Biotechnology, USA) at 4°C overnight. ..

    Article Title: Tie2-mediated vascular remodeling by ferritin-based protein C nanoparticles confers antitumor and anti-metastatic activities
    Article Snippet: For immunohistochemical (IHC) analysis, the harvested tumor or tissue samples were fixed in 3.7% formaldehyde (FA), dehydrated by serially incubating with 10% to 40% sucrose solution, and finally embedded in tissue freezing medium (Leica, Wetzlar, Germany). .. The frozen blocks were cut into 5 to 10 μm sample sections, which were blocked with 5% BSA in PBST (0.3% Triton X-100 in phosphate-buffered saline (PBS)) and incubated overnight at 4 °C with the appropriate primary antibody: anti-CD31 (BD Biosciences, CA, USA), anti-CD31 (Milipore, MA, USA), anti-α-SMA (Abcam, Cambridge, UK), antipimonidazole (Hypoxyprobe-1, HPI, MA, USA), anticytokeratin II (Millipore, MA, USA), anti-LYVE-1 (Angiobio, CA, USA), anti-Phospho-Tie-2 (R&D systems, MN, USA), anti-PAR1 (Abcam, Cambridge, UK), anti-PAR3 (Santa Cruz, CA, USA), anti-CD68 (Abcam, Cambridge, UK), anti-iNOS (Abcam, Cambridge, UK), anti-arginase1 (BD Biosciences, CA, USA), anti-CD4, PE (Invitrogen, CA, USA), or Alexa Fluor 647 anti-CD8a (BioLegend, CA, USA). .. After a few washes, the samples were incubated at RT for 1 h with the recommended fluorophore-tagged secondary antibody, their nuclei stained with Antifade Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA) or Hoechst 33258 (Invitrogen, USA), and mounted with fluorescent mounting medium (Sigma, USA).

    Article Title: Crumbs2 mediates ventricular layer remodelling to form the adult spinal cord central canal
    Article Snippet: .. Tissues were fixed in 4% paraformaldehyde for 2 hrs, transferred to 30% sucrose overnight, then cryosectioned at 15μm and incubated with primary antibodies: anti-Nestin (1:300, AbCAM), anti-Nkx6.1 (1:50, DSHB), anti-Sox1 (1:300, Cell Signalling Technologies), anti-Sox2 (1:1000, Millipore), anti-Sox3 (1:1000 Gift from T.Edlund), anti-Zo-1 (1:300, Zymed) anti-Phalloidin (1:500, Thermofisher), anti-aPKC (1:300, Santa-Cruz), anti-PAR3 (1:200, Millipore), anti-CRB2 (1:300, ), anti-Dystroglycan (1:30, Gift from S.Winder), anti-M2 (1:50, DSHB), anti-Transitin (1:50, DSHB), anti-Pax6 (1:50, DSHB) and anti-pH3 (1:1000, Millipore). .. Alexa 488 and 594 conjugated secondary antibodies were used (1:500; ThermoFisher Scientific/Molecular Probes, cat. nos.

    Western Blot:


    other:

    Article Title: ALOX15 mediates thrombin-induced myristoylation, trafficking, and interaction of protease-activated receptors, leading to platelet activation and hemostasis.
    Article Snippet: Anti-b-actin (sc-47 778), anti-eIF4E (sc-9979), anti-MEK1 (sc-219), anti-PAR1 (sc-13 503), anti-PAR3 (sc-393 127), anti-PAR4 (sc-25 466), anti-PKCa (sc-208), anti-PKCd (sc937), anti-PKCc (sc-211), anti-PKCf (sc-216), anti-4EBP1 (sc-6936), and anti-a-tubulin (sc-23 948) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Saline:

    Article Title: Tie2-mediated vascular remodeling by ferritin-based protein C nanoparticles confers antitumor and anti-metastatic activities
    Article Snippet: For immunohistochemical (IHC) analysis, the harvested tumor or tissue samples were fixed in 3.7% formaldehyde (FA), dehydrated by serially incubating with 10% to 40% sucrose solution, and finally embedded in tissue freezing medium (Leica, Wetzlar, Germany). .. The frozen blocks were cut into 5 to 10 μm sample sections, which were blocked with 5% BSA in PBST (0.3% Triton X-100 in phosphate-buffered saline (PBS)) and incubated overnight at 4 °C with the appropriate primary antibody: anti-CD31 (BD Biosciences, CA, USA), anti-CD31 (Milipore, MA, USA), anti-α-SMA (Abcam, Cambridge, UK), antipimonidazole (Hypoxyprobe-1, HPI, MA, USA), anticytokeratin II (Millipore, MA, USA), anti-LYVE-1 (Angiobio, CA, USA), anti-Phospho-Tie-2 (R&D systems, MN, USA), anti-PAR1 (Abcam, Cambridge, UK), anti-PAR3 (Santa Cruz, CA, USA), anti-CD68 (Abcam, Cambridge, UK), anti-iNOS (Abcam, Cambridge, UK), anti-arginase1 (BD Biosciences, CA, USA), anti-CD4, PE (Invitrogen, CA, USA), or Alexa Fluor 647 anti-CD8a (BioLegend, CA, USA). .. After a few washes, the samples were incubated at RT for 1 h with the recommended fluorophore-tagged secondary antibody, their nuclei stained with Antifade Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA) or Hoechst 33258 (Invitrogen, USA), and mounted with fluorescent mounting medium (Sigma, USA).



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    a) Simplified phylogeny of animals shows that acoel brains are likely intermediate between cnidarian diffuse nets and the centralized brains of typical bilaterians. b) Photograph of juvenile Hofstenia miamia . c) Staining with voltage dye reveals a superficial network of dense neuropil (blue arrow) that extends into a sparser posterior nerve net (green arrow). d) Close-up view of neuropil stained sparsely with tubulin dye (orange) reveals that the neuropil (orange) contains many neurites running in parallel, with cellular clusters (cyan) interspersed between neurite bundles. Sensory neurons (likely clusters of H1 cells; bright orange) are set within many of these patches. e) Cross-section of brain stained with a <t>Par3</t> antibody reveals that the brain has two layers: superficial neuropil, and deeper cell bodies that project outward. f) Staining with an ERK antibody (z-projected segmentation overlaid) shows that brain interneurons can be multipolar, with a central cell body generating multiple neurites. g) Cross-section of brain stained with an antibody against β-catenin reveals another sensory neuron class (possibly H2 ) with two projections that innervate brain neuropil. h) Electron microscopy cross-section shows the fine organization of the brain, confirming the relative configuration of tissue types within the head. The superficial neuropil (previously ‘layer 1’) is visible immediately beneath the skin, while neural cell bodies (previously ‘layer 2’) lie deeper in the tissue, internal to body wall muscle (green). Together, these layers compose the brain. i) Electron microscopy close-up of the brain shows dense neuropil; the box is a 6.7×6.7µm square. j) Segmenting neural projections within the highlighted box in (i) reveals over 400 neurites in a single section of neuropil. k) Segmentation of cellular clusters within neuropil allows quantification of brain structure and its variability. l) Quantifying the numbers of cellular clusters across brains reveals that, although cluster numbers increase with age (i.e. days after hatching) and size (i.e. head width, a good proxy for overall body size ), worms vary widely in how many clusters they possess. Linear regression p<0.0001, n=49. Scale bars: 200µm (c), 50µm (d,e), 20µm (f,g), 10µm (h).
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    a) Simplified phylogeny of animals shows that acoel brains are likely intermediate between cnidarian diffuse nets and the centralized brains of typical bilaterians. b) Photograph of juvenile Hofstenia miamia . c) Staining with voltage dye reveals a superficial network of dense neuropil (blue arrow) that extends into a sparser posterior nerve net (green arrow). d) Close-up view of neuropil stained sparsely with tubulin dye (orange) reveals that the neuropil (orange) contains many neurites running in parallel, with cellular clusters (cyan) interspersed between neurite bundles. Sensory neurons (likely clusters of H1 cells; bright orange) are set within many of these patches. e) Cross-section of brain stained with a <t>Par3</t> antibody reveals that the brain has two layers: superficial neuropil, and deeper cell bodies that project outward. f) Staining with an ERK antibody (z-projected segmentation overlaid) shows that brain interneurons can be multipolar, with a central cell body generating multiple neurites. g) Cross-section of brain stained with an antibody against β-catenin reveals another sensory neuron class (possibly H2 ) with two projections that innervate brain neuropil. h) Electron microscopy cross-section shows the fine organization of the brain, confirming the relative configuration of tissue types within the head. The superficial neuropil (previously ‘layer 1’) is visible immediately beneath the skin, while neural cell bodies (previously ‘layer 2’) lie deeper in the tissue, internal to body wall muscle (green). Together, these layers compose the brain. i) Electron microscopy close-up of the brain shows dense neuropil; the box is a 6.7×6.7µm square. j) Segmenting neural projections within the highlighted box in (i) reveals over 400 neurites in a single section of neuropil. k) Segmentation of cellular clusters within neuropil allows quantification of brain structure and its variability. l) Quantifying the numbers of cellular clusters across brains reveals that, although cluster numbers increase with age (i.e. days after hatching) and size (i.e. head width, a good proxy for overall body size ), worms vary widely in how many clusters they possess. Linear regression p<0.0001, n=49. Scale bars: 200µm (c), 50µm (d,e), 20µm (f,g), 10µm (h).
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    Merck KGaA antibody anti-par3 (rabbit polyclonal)
    a) Simplified phylogeny of animals shows that acoel brains are likely intermediate between cnidarian diffuse nets and the centralized brains of typical bilaterians. b) Photograph of juvenile Hofstenia miamia . c) Staining with voltage dye reveals a superficial network of dense neuropil (blue arrow) that extends into a sparser posterior nerve net (green arrow). d) Close-up view of neuropil stained sparsely with tubulin dye (orange) reveals that the neuropil (orange) contains many neurites running in parallel, with cellular clusters (cyan) interspersed between neurite bundles. Sensory neurons (likely clusters of H1 cells; bright orange) are set within many of these patches. e) Cross-section of brain stained with a <t>Par3</t> antibody reveals that the brain has two layers: superficial neuropil, and deeper cell bodies that project outward. f) Staining with an ERK antibody (z-projected segmentation overlaid) shows that brain interneurons can be multipolar, with a central cell body generating multiple neurites. g) Cross-section of brain stained with an antibody against β-catenin reveals another sensory neuron class (possibly H2 ) with two projections that innervate brain neuropil. h) Electron microscopy cross-section shows the fine organization of the brain, confirming the relative configuration of tissue types within the head. The superficial neuropil (previously ‘layer 1’) is visible immediately beneath the skin, while neural cell bodies (previously ‘layer 2’) lie deeper in the tissue, internal to body wall muscle (green). Together, these layers compose the brain. i) Electron microscopy close-up of the brain shows dense neuropil; the box is a 6.7×6.7µm square. j) Segmenting neural projections within the highlighted box in (i) reveals over 400 neurites in a single section of neuropil. k) Segmentation of cellular clusters within neuropil allows quantification of brain structure and its variability. l) Quantifying the numbers of cellular clusters across brains reveals that, although cluster numbers increase with age (i.e. days after hatching) and size (i.e. head width, a good proxy for overall body size ), worms vary widely in how many clusters they possess. Linear regression p<0.0001, n=49. Scale bars: 200µm (c), 50µm (d,e), 20µm (f,g), 10µm (h).
    Antibody Anti Par3 (Rabbit Polyclonal), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a) Simplified phylogeny of animals shows that acoel brains are likely intermediate between cnidarian diffuse nets and the centralized brains of typical bilaterians. b) Photograph of juvenile Hofstenia miamia . c) Staining with voltage dye reveals a superficial network of dense neuropil (blue arrow) that extends into a sparser posterior nerve net (green arrow). d) Close-up view of neuropil stained sparsely with tubulin dye (orange) reveals that the neuropil (orange) contains many neurites running in parallel, with cellular clusters (cyan) interspersed between neurite bundles. Sensory neurons (likely clusters of H1 cells; bright orange) are set within many of these patches. e) Cross-section of brain stained with a <t>Par3</t> antibody reveals that the brain has two layers: superficial neuropil, and deeper cell bodies that project outward. f) Staining with an ERK antibody (z-projected segmentation overlaid) shows that brain interneurons can be multipolar, with a central cell body generating multiple neurites. g) Cross-section of brain stained with an antibody against β-catenin reveals another sensory neuron class (possibly H2 ) with two projections that innervate brain neuropil. h) Electron microscopy cross-section shows the fine organization of the brain, confirming the relative configuration of tissue types within the head. The superficial neuropil (previously ‘layer 1’) is visible immediately beneath the skin, while neural cell bodies (previously ‘layer 2’) lie deeper in the tissue, internal to body wall muscle (green). Together, these layers compose the brain. i) Electron microscopy close-up of the brain shows dense neuropil; the box is a 6.7×6.7µm square. j) Segmenting neural projections within the highlighted box in (i) reveals over 400 neurites in a single section of neuropil. k) Segmentation of cellular clusters within neuropil allows quantification of brain structure and its variability. l) Quantifying the numbers of cellular clusters across brains reveals that, although cluster numbers increase with age (i.e. days after hatching) and size (i.e. head width, a good proxy for overall body size ), worms vary widely in how many clusters they possess. Linear regression p<0.0001, n=49. Scale bars: 200µm (c), 50µm (d,e), 20µm (f,g), 10µm (h).
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    Novus Biologicals par3
    Summary of distinct pathological characteristics observed in retina of two HD mouse models. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer; IPL: inner plexiform layer; ARR3: cone arrestin-3; ARR1: rod arrestin; GNAT1: rod transducin alpha-subunit; OLM: outer limiting membrane; ZO-1: tight junction protein, zonula occludens-1; Crb2: crumbs cell polarity complex component 2; <t> Par3: </t> cell polarity protein, partitioning-defective 3.
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    Image Search Results


    a) Simplified phylogeny of animals shows that acoel brains are likely intermediate between cnidarian diffuse nets and the centralized brains of typical bilaterians. b) Photograph of juvenile Hofstenia miamia . c) Staining with voltage dye reveals a superficial network of dense neuropil (blue arrow) that extends into a sparser posterior nerve net (green arrow). d) Close-up view of neuropil stained sparsely with tubulin dye (orange) reveals that the neuropil (orange) contains many neurites running in parallel, with cellular clusters (cyan) interspersed between neurite bundles. Sensory neurons (likely clusters of H1 cells; bright orange) are set within many of these patches. e) Cross-section of brain stained with a Par3 antibody reveals that the brain has two layers: superficial neuropil, and deeper cell bodies that project outward. f) Staining with an ERK antibody (z-projected segmentation overlaid) shows that brain interneurons can be multipolar, with a central cell body generating multiple neurites. g) Cross-section of brain stained with an antibody against β-catenin reveals another sensory neuron class (possibly H2 ) with two projections that innervate brain neuropil. h) Electron microscopy cross-section shows the fine organization of the brain, confirming the relative configuration of tissue types within the head. The superficial neuropil (previously ‘layer 1’) is visible immediately beneath the skin, while neural cell bodies (previously ‘layer 2’) lie deeper in the tissue, internal to body wall muscle (green). Together, these layers compose the brain. i) Electron microscopy close-up of the brain shows dense neuropil; the box is a 6.7×6.7µm square. j) Segmenting neural projections within the highlighted box in (i) reveals over 400 neurites in a single section of neuropil. k) Segmentation of cellular clusters within neuropil allows quantification of brain structure and its variability. l) Quantifying the numbers of cellular clusters across brains reveals that, although cluster numbers increase with age (i.e. days after hatching) and size (i.e. head width, a good proxy for overall body size ), worms vary widely in how many clusters they possess. Linear regression p<0.0001, n=49. Scale bars: 200µm (c), 50µm (d,e), 20µm (f,g), 10µm (h).

    Journal: bioRxiv

    Article Title: Distributed neural computation and the evolution of the first brains

    doi: 10.1101/2025.10.03.680388

    Figure Lengend Snippet: a) Simplified phylogeny of animals shows that acoel brains are likely intermediate between cnidarian diffuse nets and the centralized brains of typical bilaterians. b) Photograph of juvenile Hofstenia miamia . c) Staining with voltage dye reveals a superficial network of dense neuropil (blue arrow) that extends into a sparser posterior nerve net (green arrow). d) Close-up view of neuropil stained sparsely with tubulin dye (orange) reveals that the neuropil (orange) contains many neurites running in parallel, with cellular clusters (cyan) interspersed between neurite bundles. Sensory neurons (likely clusters of H1 cells; bright orange) are set within many of these patches. e) Cross-section of brain stained with a Par3 antibody reveals that the brain has two layers: superficial neuropil, and deeper cell bodies that project outward. f) Staining with an ERK antibody (z-projected segmentation overlaid) shows that brain interneurons can be multipolar, with a central cell body generating multiple neurites. g) Cross-section of brain stained with an antibody against β-catenin reveals another sensory neuron class (possibly H2 ) with two projections that innervate brain neuropil. h) Electron microscopy cross-section shows the fine organization of the brain, confirming the relative configuration of tissue types within the head. The superficial neuropil (previously ‘layer 1’) is visible immediately beneath the skin, while neural cell bodies (previously ‘layer 2’) lie deeper in the tissue, internal to body wall muscle (green). Together, these layers compose the brain. i) Electron microscopy close-up of the brain shows dense neuropil; the box is a 6.7×6.7µm square. j) Segmenting neural projections within the highlighted box in (i) reveals over 400 neurites in a single section of neuropil. k) Segmentation of cellular clusters within neuropil allows quantification of brain structure and its variability. l) Quantifying the numbers of cellular clusters across brains reveals that, although cluster numbers increase with age (i.e. days after hatching) and size (i.e. head width, a good proxy for overall body size ), worms vary widely in how many clusters they possess. Linear regression p<0.0001, n=49. Scale bars: 200µm (c), 50µm (d,e), 20µm (f,g), 10µm (h).

    Article Snippet: Primary antibodies used: Par3 (St. John’s Laboratory #STJ94951, 1:200), pERK (Cell Signaling Technologies #4370T, 1:200) , FMRFamide (EMDMillipore #AB15348, 1:1000) .

    Techniques: Staining, Electron Microscopy

    Summary of distinct pathological characteristics observed in retina of two HD mouse models. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer; IPL: inner plexiform layer; ARR3: cone arrestin-3; ARR1: rod arrestin; GNAT1: rod transducin alpha-subunit; OLM: outer limiting membrane; ZO-1: tight junction protein, zonula occludens-1; Crb2: crumbs cell polarity complex component 2;  Par3:  cell polarity protein, partitioning-defective 3.

    Journal: Neurobiology of disease

    Article Title: Pleiotropic effects of mutant huntingtin on retinopathy in two mouse models of Huntington’s disease

    doi: 10.1016/j.nbd.2024.106780

    Figure Lengend Snippet: Summary of distinct pathological characteristics observed in retina of two HD mouse models. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer; IPL: inner plexiform layer; ARR3: cone arrestin-3; ARR1: rod arrestin; GNAT1: rod transducin alpha-subunit; OLM: outer limiting membrane; ZO-1: tight junction protein, zonula occludens-1; Crb2: crumbs cell polarity complex component 2; Par3: cell polarity protein, partitioning-defective 3.

    Article Snippet: Primary antibodies against the following proteins were used: PHP1 (1:800, MABN2490, MilliporeSigma), cone arrestin Lumi-J (1:500, gift from Dr. Cheryl Craft, University of Southern California), ZO-1 (1:500, 61–7300, ThermoFisher Scientific), Crb2 (1:400, BS-14046R, ThermoFisher), Par3 (1:400, NBP1–88861, Novus Biologicals), Centrin 2 (1:200, 15,877–1-AP, Proteintech), GNAT1 (1:2000, PA5–28336, ThermoFisher), rabbit C10C10 antibody against ARR1(1:500) , mouse monoclonal anti-rhodopsin antibody 1D4 ( ) (sc-57,432, Santa Cruz Biotechnology).

    Techniques: Membrane, Transgenic Assay, Knock-In, Labeling, Expressing, Translocation Assay, Disruption, Staining